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KMID : 0438219730100030807
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Korea University Medical Journal
1973 Volume.10 No. 3 p.807 ~ p.815
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Studies on Phytohemoagglutinin in Laccaria laccata B. Native to Korea
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Abstract
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The author screened twenty three kinds of mushroom native to Korea on its hemoagglutination activity to human red blood cell (RBC) and found out a new Phytohemoagglutinin (PHA) in the saline extract of Laccaria laccata B. (LLB).
Its hemoagglutination titer, absorption test with human saliva and sugars, heat stability test, salt fractionation by ammonium sulfate, sperm agglutination activity and elution test were studied. The results could be summarized as follows:
1) Anti A-lectin was found in LLB.
2) Anti A-lectin in LLB was absorbed by same group´s RBC and saliva of secretor.
3) The agglutination titer of anti A-lectin in LLB were 1:8 with type A RBC and 1:4 with type AB RBC, and its optimal temperature of agglutination was 37˚C.
4) The anti A-lectin was found in precipitates of LLB treated by 40% saturated ammonium sulfate solution.
5) The agglutinating activity of anti A-fraction in LLB was inhibited by various sugars except dextrose.
6) The human sperms were not agglutinated by PHA in LLB.
7) It was possible to detect blood type A RBC in the blood stains and various humoral stains by elution test with anti A-lectin in LLB.
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